fructose-1,6-diphosphate aldolase in serum

Indications

• none really
• AST, LDH & CK have replaced aldolase

Reference interval

1.0-7.5 U/L (adult)

Principle

-..........-

                         aldolase
fructose-1,6-diphosphate -------> glyceraldehyde-3-phosphate
                                  + dihydroxyacetone phosphate

                       triose phosphate
                          isomerase
dihydroxyacetone phosphate -------> glyceraldehyde-3-phosphate

                           glyceraldehyde-
                           3-phosphate
                           dehydrogenase
glyceraldehyde-3-phosphate -----------> 1,3-diphosphoglycerate
+  NAD+  +  H2PO4-                      + NADH

production of NADH is monitored at 340 nM

Clinical significance

• serum creatine kinase generally sufficient
• serum aldolase monitoring not routinely recommended
• increased serum aldolase in the absence of increased serum creatine kinase occurs in eosinophilic fasciitis

Increases

• Duchenne muscular dystrophy (highest levels)
• dermatomyositis, polymositis
• limb-girdle dystrophy
• myocardial infarction
• viral hepatitis
• cirrhosis
• obstructive jaundice
• prostate cancer
• chronic leukemia

Specimen

• serum, avoid hemolysis
• separate from cells immediately
• stable at room temperature for 8 hours
• stable at 4 degrees C for 5 days

More general terms

• aldolase measurement

Additional terms

• fructose-1,6 diphosphate aldolase or fructose diphosphate aldolase

Patient information

aldolase in serum patient information